FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l01n01 se380.34100000007f82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l01n01 se380.34100000007f82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31497627
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC1521120.48293161894386527TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG198450.037.90446528
CGTATGC198450.037.54882444
GCACACG205400.037.4126111
CGTGGCC197250.037.1574933
GTATGCC201200.036.93490645
GTCACGT203900.036.55969629
ACACGTC211350.036.42321813
CACACGT212050.036.37724712
GTGGCCA201250.036.30850234
ACGTCTG212350.036.1457615
CTCGTAT206500.036.01825742
GGCCATC205300.035.8664736
TCGTATG211250.035.33651443
CACGTCT217700.035.31947314
TGGCCAT210050.034.78763635
CAGTCAC216500.034.78732327
CGTCTGA222150.034.6119716
GCCATCT217800.033.99454537
CCAGTCA222800.033.753226
TCACGTG221800.033.5787730
Produced by FastQC (version 0.11.2)