FastQCFastQC Report
Wed 17 Sep 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277849
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC166580.5081991269274454No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA73260.22350022835097041No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA49450.15086112874632115No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA44590.13603433227094966No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC42090.12840737935151986No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG41720.12727859031944425No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT35390.10796714552744804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC22800.035.8210515
GACCATA22900.035.756618
GTCGTAG23750.034.5492027
GTAGTTC23800.034.38556710
TAGTTCC23950.034.36111511
ACGATGC24300.033.4472526
ACCGTCG24600.033.2642944
CCGTCGT24800.032.9960335
AACGATG24700.032.90055525
GGTATAG4950.032.6132622
CGACCGG25050.032.2468433
TAAACGA25450.032.1078923
CCGGCGA25400.031.91582936
AGTTCCG25800.031.81080812
TATAGGG5050.031.5200964
TCGAACC5000.031.51526823
CCGACCG25700.031.41968532
TCGCGAC502.026709E-631.4123061
TTTCGAG11400.031.09950837
ATAGGGG5150.030.9080545
Produced by FastQC (version 0.11.2)