FastQCFastQC Report
Wed 17 Sep 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424268
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA128320.37473702408806786No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT62100.18135262777329345No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC51800.15127320641959097No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG44940.1312397277315911No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA37480.1094540497414338No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG37420.10927882981121805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA16500.037.4983545
GCCCTGT16900.036.3409144
TCGAACC5050.035.24154323
GGTATAG5050.034.6562232
TATAGGG5000.034.1022874
CATCTAG5300.034.00176629
ATCTAGT5300.033.586130
TTCGAGG18900.033.0567138
TAATCGA5450.033.02042820
TTTCGAG19300.032.5995837
ATAGGGG5250.032.478375
TCTAGTA5550.032.07885731
CTTTCGA20200.030.9261236
CTAGTAG5850.030.82580632
CAATACA20700.030.79890424
GACTCTT20700.030.49074432
AGGCCCT20450.030.47578842
AGGGGCG5700.030.3078947
ATACAGG20900.030.18258326
AGGTAGT21250.029.9846745
Produced by FastQC (version 0.11.2)