FastQCFastQC Report
Wed 17 Sep 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167112
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA336460.8074177031958825No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG269570.646898859449902No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC145930.3501945712042297No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG86590.20779379099961798No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG73600.17662112273440214No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG62620.1502719389351666No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA56300.135105559917756No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT51780.1242587192280889No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA50450.12106706035258952No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT47760.11461175029612834No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA43990.10556471724302105No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA42580.10218107888628865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC39250.032.9238936
ATTACGC352.7373558E-432.3094141
GGTGTGC47650.031.6790397
TTGATCC41100.031.3329327
CCTGGTT41900.031.1641752
AGCCATG40150.031.0871345
GTGTGCT71000.030.029888
TGATCCT43000.029.9484548
GTGCTCT62400.029.9334310
CATATGC43450.029.76710923
GCATATG44100.029.37095322
TAAGTAA7850.029.30761130
GCTTGTC43900.029.27080528
GCCAGTA44300.029.24092115
GATCCTG44200.029.0340339
ATCCTGC44700.028.90968110
TGCCAGT44850.028.78239614
CTTGTCT45000.028.60884929
GTCTCAA45050.028.36008832
TCCTGCC45650.028.2160311
Produced by FastQC (version 0.11.2)