FastQCFastQC Report
Wed 17 Sep 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695398
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC251400.6803056125483642No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA77310.2092061531667225No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA75570.2044975940345262No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG69950.18928948925122543No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA51070.13819891659842864No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC41580.11251832684869127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC35050.035.02995315
TACCCGT458.8474735E-734.9283941
GACCATA35400.034.80524418
AACGATG35550.034.4555725
ACGATGC35800.034.15252726
TAAACGA36500.033.55878423
GTTGATC11400.033.46366
CGACCGG36300.033.4100633
CCGGCGA36150.033.30740436
CCGACCG36900.032.80571732
CGTTTCG551.1103111E-732.78905533
GTCGTAG38650.031.817037
TAGTTCC38450.031.8143311
GTAGTTC38500.031.76613610
CGATGCG38650.031.30200440
CCCTGTA11550.031.26549545
CGATGCC39400.031.26313627
AGTTCCG39300.031.12623412
TGCGGCG39250.031.00277343
ACCGTCG39550.030.9795254
Produced by FastQC (version 0.11.2)