FastQCFastQC Report
Wed 17 Sep 2014
MC10_CTTGTA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC10_CTTGTA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9998761
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA749970.7500629327973736No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT492390.49245101468071895No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG353460.35350379912071106No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC325110.3251502861204503No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA311240.31127856741450266No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG275600.2756341510713177No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC227900.22792824030897427No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT218170.2181970346125885No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGA208540.20856584130773803No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT205170.20519542371299804No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA201660.2016849887701086No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT199880.19990476820078007No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA198620.19864461206743514No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT190010.19003354515624488No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC186560.18658311764827662No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA172750.17277140637725014No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC135970.13598684877056266No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG135810.13582682894410616No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG135150.13516674715997312No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA131530.13154629858639486No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG129100.1291159974720868No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC127680.12769582151228537No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC122200.12221514245615032No Hit
TAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTG119120.11913476079686275No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC111010.111023755843349No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG110460.11047368768990477No Hit
AGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAA105790.10580310900520574No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG105070.10508301978615152No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG104970.1049830073946162No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC100620.10063246836283016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA102400.036.25343745
TCGAACC49200.035.48514623
ATCGAAC49900.035.07753422
CATCTAG52250.033.6300729
GCCCTGT110450.033.56957244
CGAACCA52100.033.46678524
TATAGGG52650.033.1171764
ATCTAGT52550.033.09556630
TTTCGAG112000.033.09251837
TCTTTCG112100.033.05787335
TCTAGTA53050.032.9532831
GGTATAG53350.032.7717482
ATAGGGG53850.032.5463075
TTCGAGG114950.032.32156438
AATCGAA54800.031.81787321
TCGAGGC116800.031.79035239
AGGGGCG55850.031.3405367
GGCGAAA56000.031.17623710
CTTTCGA118900.031.11531436
TAATCGA56050.031.06814620
Produced by FastQC (version 0.11.2)