FastQCFastQC Report
Wed 17 Sep 2014
MC09_GGCTAC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC09_GGCTAC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9472510
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA486720.513823685591253No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT349380.368835715137804No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT302320.31915511305873523No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC290320.3064868762344933No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG226880.23951413089033424No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT222100.2344679498886779No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC197120.20809690356621424No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC191240.20188946752233566No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA177480.18736322263053828No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA168280.17765090773195277No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC163280.17237247572185196No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGA142410.1503403005116912No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG141980.14988635535882253No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG133010.14041684833270168No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT129340.13654247923728768No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT120910.12764304286825773No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG118720.12533108964783357No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG116540.12302969329142963No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA106400.11232503317494519No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA104200.11000252309050083No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC102910.10864068763189483No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA96230.10158870246640013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA69200.033.650145
TCGAACC35000.031.0471723
TTTCGAG77100.030.26963437
GCCCTGT76900.030.25113744
ATCGAAC36150.029.99726322
TCTTTCG77550.029.73946435
CGAACCA36900.029.57047524
TTCGAGG79200.029.32495138
TCTAGTA38050.028.97361431
CATCTAG38700.028.71937629
ATCTAGT38600.028.44405630
GGCGAAA38700.028.4276410
ACAATAC82650.027.92849223
GGTATAG39700.027.6597292
TATAGGG39550.027.646174
CTTTCGA84200.027.47665236
CAATACA84150.027.45739224
AATCGAA39850.027.43789921
CTAGTAG42350.026.67028432
AGGGGCG41300.026.6380067
Produced by FastQC (version 0.11.2)