FastQCFastQC Report
Wed 17 Sep 2014
MC08_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC08_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19084481
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA2767561.4501625692624285No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT890330.4665204152001828No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC845260.44290436821415263No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG789750.4138179078592706No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG735050.3851558761278339No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT716500.37543593666497926No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC661980.34686822240541937No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA638210.33441307625813876No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT573610.30056358357348045No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC559570.2932068207670934No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA556540.2916191433238347No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA485250.2542641846010903No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC478240.25059104305744545No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG456890.23940394292095235No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT455630.23874372061781507No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC440980.2310673263789568No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC417110.21855978163618908No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG339880.17809234634151172No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG338880.17756836038664087No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA329540.17267433156814693No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG316330.16575247710430271No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC307250.16099468463407518No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA293930.15401519171519518No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGA285850.14978138519983855No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG282110.14782167772862148No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG274350.1437555467188235No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT252140.13211781866114147No Hit
GCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG250670.1313475593074813No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG248970.13045678318420081No Hit
CGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTG247490.12968128397099193No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCT247270.12956600706092034No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT233700.12245551765332262No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA232720.12194201141754916No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGC228610.11978842914302987No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA210240.11016280715205197No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCG207370.10865896746157257No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA199490.10452995813719011No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGA198010.10375445892398122No Hit
CGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCG194110.10171091369998483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA344800.039.17742545
GCCCTGT363750.037.2168444
TCGAACC96450.035.36374723
TTTCGAG380750.035.2937737
TCTTTCG381950.035.06828735
TTCGAGG383750.035.06477438
TCGAGGC387350.034.8197539
ATCGAAC98500.034.6734322
TCTAGTA99650.034.36404831
CGAACCA99450.034.1838524
TATAGGG100350.034.1238944
CTTTCGA395250.033.9705336
AGGCCCT398950.033.90884442
GGTATAG101350.033.7009622
ACAATAC402150.033.6350623
ATCTAGT102200.033.50645430
CAATACA403550.033.4960724
CATCTAG101900.033.494729
ATACAGG406250.033.24039526
CGAGGCC408900.033.221340
Produced by FastQC (version 0.11.2)