FastQCFastQC Report
Wed 17 Sep 2014
MC07_GGCTAC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC07_GGCTAC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18281362
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA2356911.289242015994213No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT961460.5259236155380546No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC800480.4378667191208182No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG744160.4070593864942885No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA605250.3310748947479953No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG592790.3242592100085322No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC504320.27586566033756127No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC436660.2388552887908461No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG433010.23685871982623616No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT430170.23530522507021084No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA422440.23107687490680398No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA421210.23040405851599022No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT408770.22359931388044285No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC399470.2185121655596558No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT390890.2138188609798329No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG347500.19008430553478456No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC337780.1847674150317684No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG316900.17334594654380783No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG306930.16789230474184583No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA296940.16242772283596812No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG294630.1611641408337081No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC290770.1590527007779836No Hit
CGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTG254290.13909795123579963No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC236650.12944877958217774No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA220290.12049977457915882No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGA218900.11973943735701968No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGC215200.11771551813262054No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG211680.11579005984346243No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA201750.11035829824933174No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG198320.10848207042779417No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA197180.10785848450460091No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT194030.10613541813788274No Hit
CGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCG189260.10352620335399518No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT187760.10270569556031985No Hit
AGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGC186610.10207663958516876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA293500.038.54305345
GCCCTGT309200.036.6805844
TCTTTCG319050.035.27620735
TTTCGAG319450.035.2067537
TTCGAGG325850.034.54978638
TCGAACC84200.034.3894623
TCGAGGC329800.034.2300139
ACAATAC335150.033.78005623
CTTTCGA334050.033.66800736
ATCGAAC86500.033.60511422
CAATACA340200.033.27200324
ATACAGG340250.033.1747226
GACTCTT340450.033.1249132
ACTCTTT341700.033.03665533
CGAACCA88550.032.75090424
TAACAAT346350.032.68770221
TGACGAA348000.032.65564311
GGACTCT346550.032.59137731
AGGCCCT348950.032.5320642
GTGACGA350300.032.3640710
Produced by FastQC (version 0.11.2)