FastQCFastQC Report
Wed 17 Sep 2014
MC05_TAGCTT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC05_TAGCTT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10365020
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA1305451.259476585669878No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG565580.5456622370241446No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG444510.4288559018699433No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA389760.376034006687879No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC372370.35925642208119235No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT356180.3436365776428796No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC324820.3133809679093721No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT318510.3072931841906721No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG311430.3004625171972654No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA306370.2955807128206217No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT265160.25582198587171084No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT233190.22497785821927985No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG220800.21302419098081815No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC217030.2093869572851765No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA216650.20902033956519137No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC190670.18395526491989403No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC188240.18161084107893666No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC185390.17886120817904838No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA155660.1501781955075822No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC153130.14773729331926036No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG142490.13747199715967745No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG140420.1354748953692323No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA136140.13134562210203163No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGC136080.13128773509361294No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT133290.12859598920214335No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG131100.1264831133948608No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA129060.12451495510862497No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGA115660.11158685656178183No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG113600.10959940260607312No Hit
AGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGC109790.10592357757148563No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCT109650.10578850788517533No Hit
GCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG108210.10439921968312652No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG106940.10317394467159735No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA104420.10074269031801193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA160150.038.72014245
GCCCTGT169300.036.5860144
TCGAACC68600.036.17362623
TTTCGAG175550.035.13780237
TCTTTCG175750.035.05500435
ATCGAAC71050.035.02125522
ATCTAGT71000.035.0162630
CATCTAG71300.034.9004829
TTCGAGG178400.034.6647738
CGAACCA71750.034.64822824
TATAGGG71900.034.638694
TCTAGTA72050.034.53735431
GGTATAG72700.034.2613222
CTTTCGA181100.034.04854236
ACAATAC182700.033.75290723
CTAGTAG74400.033.75016432
ATAGGGG74100.033.7012065
TCGAGGC184650.033.5767539
CAATACA183550.033.57208624
CTAATCG74400.033.47572719
Produced by FastQC (version 0.11.2)