FastQCFastQC Report
Wed 17 Sep 2014
MC04_GATCAG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC04_GATCAG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10881715
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA753010.6919957010452855No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT490590.4508388613375741No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT389480.35792152248060166No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG305640.2808748437171898No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT300860.2764821537781499No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA272890.2507784848252321No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC268790.24701069638379614No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA234680.21566453449663034No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG230430.2117589001366053No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC221150.20323083263989178No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC201010.184722720637326No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG179720.16515778992557698No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT179590.16503832346279973No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA168450.15480096657558115No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG167280.153725768410586No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC160030.14706321567877859No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC159610.14667724710672905No Hit
ACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA153020.14062121641671374No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA150890.13866380437274822No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA150380.13819512824954522No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC149000.13692694579852532No Hit
ACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACT148830.13677072042412433No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG145490.13370135130353994No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG143070.13147743715030213No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG139220.12793939190651474No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGA130110.11956754978420221No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA128310.11791339876113277No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG124810.11469699399405332No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC121150.11133355358047882No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGA111790.10273196826051775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA107900.035.5744145
GCCCTGT117600.032.63907644
TCGAACC47700.032.54459823
ATCGAAC49900.031.19993822
TTTCGAG122000.031.17330437
TCTTTCG121750.031.1040735
CGAACCA50100.030.94066824
TTCGAGG124100.030.55512638
TATAGGG51600.030.0849694
TCTAGTA52350.029.99914431
CTTTCGA127350.029.7223436
ATCTAGT52850.029.67248730
CATCTAG52600.029.59964629
GGTATAG52400.029.4579732
ACAATAC129600.029.42475723
CTAATCG53400.029.15540119
CAATACA130900.029.13253224
CTAGTAG54600.029.05243932
AATCGAA54050.028.88763221
ATAGGGG54350.028.7695755
Produced by FastQC (version 0.11.2)