FastQCFastQC Report
Wed 17 Sep 2014
MC03_TAGCTT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMC03_TAGCTT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23806981
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT1630910.6850553625426088No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA1428470.6000214810941379No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC1169240.49113325204905234No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT1160550.48748306221607857No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG1088920.4573952488977918No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA742890.3120471260089635No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG734380.3084725442507809No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT627030.26338072853504607No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA618910.25996996427224434No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG614670.2581889740660523No Hit
AGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTG611410.2568196278226122No Hit
AGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGA517540.2173900168190162No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG495790.208254041115083No Hit
CAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGA472310.19839138780343465No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA461890.1940145203627457No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGA453920.1906667628289366No Hit
TGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC429940.18059408708731275No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC428650.180052229217976No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG425180.1785946735539462No Hit
CGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTG420250.176523852394388No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACC375390.15768063997698825No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG349780.14692329111364436No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAA345810.14525571301963908No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC343150.14413839369216955No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG323060.1356996924557549No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT322190.13533425342759756No Hit
CCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGC316260.1328433874080884No Hit
GGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG313330.1316126559684321No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG294900.12387122919953605No Hit
AAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTT283540.11909951959049322No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG276530.11615500512223705No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTT263850.11082883629805897No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC263810.11081203450366092No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTG253310.10640156347417591No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGT245580.10315461670675505No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA240580.10105439240700029No Hit
GCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG238210.10005888608891653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA195700.034.98418445
GCCCTGT215350.031.79185544
TCGAACC79350.030.5651823
TCTAGTA82650.029.39979231
ATCGAAC83400.029.05392322
CGAACCA84200.028.77787624
ATCTAGT84850.028.61075430
CTAGTAG87050.028.1999632
CATCTAG86250.028.19851729
CTAATCG86300.027.94842519
TTTCGAG245550.027.68498837
TCTTTCG246800.027.4418835
TATAGGG89600.027.1188264
TTCGAGG254050.026.7764238
GGTATAG90650.026.7323862
CCCGTCC84700.026.16824340
ATAGGGG93350.026.0294255
AATCGAA93050.026.0166321
CCGTCCC86100.025.82114441
ACAATAC266650.025.742823
Produced by FastQC (version 0.11.2)