ANUT_12_02_ACAGTG_L002_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ANUT_12_02_ACAGTG_L002_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13037831
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

$Duplication level graph$

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC	325326	2.49524633353508	No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA	92903	0.7125648430325566	No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG	92340	0.7082466401044775	No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC	63530	0.4872743019908757	No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA	61708	0.4732995848772698	No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA	60564	0.4645251192472122	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA	53444	0.40991480868251784	No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	28977	0.22225322601589176	No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA	26850	0.20593916273343318	No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT	26781	0.20540993360015175	No Hit
GCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA	24331	0.1866184643749409	No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAA	19880	0.15247935028456805	No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG	19703	0.15112176250788953	No Hit
GGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTG	19629	0.15055418343741378	No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGC	19176	0.1470796791276095	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	17741	0.13607324715284314	TruSeq Adapter, Index 5 (100% over 50bp)
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG	17011	0.1304741563224742	No Hit
TGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAA	16335	0.12528924481380377	No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA	15866	0.12169202070497769	No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG	15708	0.12048016268963757	No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGT	15646	0.12000462346842815	No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG	15586	0.11954442422209646	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT	15378	0.11794906683481325	No Hit
GAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTT	14517	0.11134520764995344	No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT	13776	0.10566174695775701	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGACCAT	42130	37.181747	17
TCCGACC	42445	36.847496	15
CCGACCA	42635	36.73078	16
TTCCGAC	42990	36.322796	14
CGACCGG	43180	36.268017	33
GACCATA	43450	36.09781	18
CGGCGAT	43470	36.09654	37
CATAAAC	43635	35.947716	21
CCGGCGA	44045	35.625305	36
CGATGCG	44145	35.523533	40
ATAAACG	44185	35.49516	22
ATGCGGC	44210	35.430176	42
ATACCGT	44045	35.29467	2
CCATAAA	44535	35.226574	20
TGCGGCG	44515	35.20891	43
AACGATG	44520	35.192963	25
GCGATGC	44695	35.076324	39
ACGATGC	44665	35.063602	26
TACCGTC	44370	35.037857	3
GATGCGG	44825	35.024395	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content