FastQCFastQC Report
Wed 17 Sep 2014
ANUT_12_01_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANUT_12_01_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20764883
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC5036232.425359198989949No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG1173470.5651223751176445No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA981350.4726007846998223No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA792030.38142762470657793No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA735730.3543145415266727No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA670590.32294427086345734No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA624910.30094559165105816No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA621710.29940452830868347No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA605770.2917281065344794No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG583410.280959926429636No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA465500.2241765580860725No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA446590.21506983689722695No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG435750.20984948482493257No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG434850.2094160607598897No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC426130.20521666315191858TruSeq Adapter, Index 4 (100% over 50bp)
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC412430.1986189857173768No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT348900.16802406254829366No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA345970.1666130264254318No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAG319000.15362475194297986No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG298050.1435356028733704No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG291780.14051608188690493No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG272420.13119264866553787No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG260950.1256688997477135No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC250150.12046781096719882No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT241610.11635509817223627No Hit
GAACCTGACCGTGGGAGGTATTCCCTTCCATGAGAAGGACCTCGTGCAGCC233760.11257467716047327No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC230360.11093729735920015No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC222770.1072820877440051No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG222050.1069353484919708No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT216630.10432517245582361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT596200.040.47104317
TTCCGAC601750.040.10525514
TCCGACC606750.039.8192615
GACCATA609300.039.68896518
CATAAAC609000.039.6696621
CCGACCA615450.039.22712716
ATAAACG618900.039.0569122
CCATAAA631900.038.28206620
TAAACGA638650.037.90193623
ACGATGC640350.037.7946526
AACGATG640750.037.77105325
CGGCGAT643150.037.67852837
CCGGCGA648400.037.3943636
CGACCGG646800.037.3864833
ACCATAA649450.037.23870519
CCGACCG656700.036.8194432
AAACGAT657350.036.79633724
CGATGCG667750.036.26659440
GACCGGC669650.036.1646234
GCCGACC671550.036.09415431
Produced by FastQC (version 0.11.2)