FastQCFastQC Report
Wed 17 Sep 2014
ANCMUT_4_02_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMUT_4_02_ACAGTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31926592
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA1822800.5709347242574466No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT1399990.438502800424173No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG1309870.410275547105059No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA787730.2467316273531481No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG605680.18971019518776072No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC561200.17577823527171332No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGA481350.15076773618681255No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT460350.14419014719767145No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG448990.14063198477306943No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG422290.13226905020116148No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA419350.13134818774268173No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA406720.1273922377934983No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG391750.12270335649981055No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG387320.12131579844162509No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC379950.11900737792495984No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA330150.1034090954649967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC440200.026.8885777
TGTGCTC464400.026.3836549
CGTATGC30100.025.33912545
TCGTATG31700.024.06040444
GTGGTGT278350.023.7551575
GTGCTCT393050.023.45706710
GTGTGCT665200.023.0800278
TCTTCCG433000.022.1000414
GCTCTTC536850.021.95194212
GCGACGC117600.021.7330447
TCTCTAG234450.021.60263618
TGCGACG54200.021.5435686
ACGCTCT306050.021.3331310
ATCTCTA242050.021.01750217
AGCGTAG140350.020.90794436
CAGCGTA175000.020.86830535
CGCTCTT283850.020.7032211
CTTCCGA416950.020.6842815
TGGTGTG382550.020.678086
CTCGACG27800.020.636926
Produced by FastQC (version 0.11.2)