FastQCFastQC Report
Wed 17 Sep 2014
ANCMUT_4_01_TGACCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMUT_4_01_TGACCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59690970
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG14095672.361440934868373No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA11202511.876751207092128No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC5742720.962075168153575No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3244360.5435260978335584TruSeq Adapter, Index 4 (100% over 50bp)
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG3139360.5259354974462637No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT2490980.417312702407081No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA2414880.4045637053644797No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTT2334690.3911295125544115No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG2150060.3601985358924474No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG2130960.3569987219172347No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA1789590.299809167115227No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1680110.281468034444741No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA1669750.27973242853986124No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC1492350.250012690361708No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT1423770.238523515365892No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA1238670.20751379982600382No Hit
TCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG1060710.1777002451124517No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA994960.1666851786794552No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA878370.1471529110684581No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG828980.13887862770532963No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA760900.1274732174732627No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA709090.11879351265358898No Hit
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTC703570.11786874966179976TruSeq Adapter, Index 4 (100% over 44bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGG642930.1077097591143183No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT639210.10708654927202557No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC625070.1047176817532032No Hit
TGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT611660.10247111078945442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC397350.039.45994645
AGCCATG1791250.037.80174345
GTTGATC1989650.034.5970886
CCTGGTT2033850.033.922032
TTGATCC2036350.033.8047757
CTGGTTG2056700.033.5112723
TGATCCT2051900.033.4981548
GATCCTG2067200.033.216489
CATATGC2068950.033.17316423
AAGCCAT2045300.033.13743644
TAGCATA2071350.033.12840320
ACCTGGT2076850.033.087721
CAGTAGC2076650.033.0829917
GTAGCAT2077100.033.05221619
GCATATG2078450.033.05184622
CCAGTAG2085300.032.9651816
TGCCAGT2080950.032.9638114
TCCTGCC2083400.032.9618811
GCCAGTA2087800.032.91385315
ATCCTGC2091750.032.90622710
Produced by FastQC (version 0.11.2)