FastQCFastQC Report
Wed 17 Sep 2014
ANCMUT_24_02_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMUT_24_02_ACAGTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17419440
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC3401131.952491010043951No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA1153660.6622830584680104No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG1029380.5909374813426839No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA906650.5204817146819875No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA741840.42586902908474666No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC678420.389461429299679No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT578600.33215763537748627No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC317620.18233651598444037No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA308950.1773593180951856No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG308170.17691154250653293No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG301260.1729447100480842No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA298360.17127990337232427No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA284840.16351845983567784No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA279080.16021180933485807No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG270870.15549868422865487No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC266450.1529612892262897No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA255310.14656613530630147No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG239300.1373752543135715No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC237280.13621563035321457TruSeq Adapter, Index 5 (100% over 50bp)
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG234790.134786192897131No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG228040.13091121184148286No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT214750.12328180469636224No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA213190.12238625351905688No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG211180.12123237027137498No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT205600.11802905259870583No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGC198570.1139933315881567No Hit
GCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA197820.1135627781375291No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA192040.11024464621135927No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG191370.10986001846213196No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG181370.10411930578709763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT445200.037.0380417
TTCCGAC451800.036.49210714
GACCATA456200.036.15955718
TCCGACC457200.036.1299915
CCGACCA459400.035.94227616
CATAAAC458800.035.91479521
ATAAACG468250.035.17556822
CCATAAA473850.034.77410520
ACCATAA481950.034.2228319
CGGCGAT483000.034.13685637
CGACCGG484100.034.06506733
AACGATG487850.033.6470525
ACGATGC488250.033.64368426
CCGGCGA491800.033.5809436
TAAACGA493150.033.36755423
ATACCGT491650.033.3276142
TACCGTC495850.033.185963
CCGACCG497250.033.1631532
TAGTTCC498750.033.11498311
CGTCGTA498950.033.0518236
Produced by FastQC (version 0.11.2)