FastQCFastQC Report
Wed 17 Sep 2014
ANCMUT_24_01_TGACCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMUT_24_01_TGACCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8147988
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC2305262.8292383346661776No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG766600.9408457646231192No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA433250.5317263599308197No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA347100.425994736369273No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA325720.39975512973264077No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA308030.3780442484696836No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA283680.34815957019082505No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC282300.3464659005388815No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA253000.3105061028563125No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG248100.3044923482950638No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA240440.29509125442992795No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG238010.29210892308628833No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA229340.28146825940342574No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC202900.2490185307096672TruSeq Adapter, Index 4 (100% over 50bp)
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT192530.23629146238310614No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA174390.21402829753799343No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG168210.2064436030097246No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG163970.2012398643689706No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA162310.19920255159924144No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGC147830.1814312932223268No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC140920.17295067199411684No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT133760.16416322655359827No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT132060.16207682190989972No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC132010.162015457067438No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG131700.16163499504417533No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA126830.15565805938840363No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG108850.13359126203916846No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG107090.13143121958451584No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAA102440.1257242892355757No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG99980.12270513898645899No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGG98970.1214655691687322No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG97200.11929325374558726No Hit
TCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC90210.11071444876943853No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG87080.10687300963133477No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT86850.10659073135601083No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC85290.10467614827120511No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG82790.10160790614811901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT274800.040.05249817
TTCCGAC278100.039.54486514
TCCGACC278250.039.51546515
GACCATA279300.039.4069418
CCGACCA280250.039.27359816
CATAAAC281050.039.23313521
ATAAACG287200.038.46351222
CCATAAA286850.038.44769720
ACCATAA291600.037.78329519
ACGATGC296200.037.1368926
CGACCGG295450.037.11784433
AACGATG296950.037.0642325
CGGCGAT298150.036.93898837
TAAACGA299100.036.90311423
CCGGCGA303300.036.3414536
AAACGAT304600.036.17030324
CCGACCG305250.035.9181532
CGTATGC31300.035.72607445
ATACCGT307900.035.6470832
TACCGTC308600.035.6066933
Produced by FastQC (version 0.11.2)