FastQCFastQC Report
Wed 17 Sep 2014
ANCMTT_4_02_CAGATC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMTT_4_02_CAGATC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23203070
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA2442181.052524515074945No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG2179680.9393929337798834No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC1671530.7203917412652723No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1381490.5953910409269119TruSeq Adapter, Index 7 (100% over 50bp)
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA741490.31956547129323837No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA731260.3151565719536251No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA703470.30317970854718795No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG636010.27410596959798855No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA590930.25467750603691663No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT477720.20588654863343514No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA471000.20299038015228155No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA465410.20058121619251243No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA459630.19809016651675834No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG458600.19764625974062916No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC452150.1948664551716648No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG451470.19457339050392902No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG438550.1890051618169492No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT435670.1877639467535977No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA435510.18769499036118928No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG425250.18327316169799945No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT408310.17597240365175815No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA347380.14971294746772731No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA346090.14915698655393445No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA334650.14422660449673255No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAG313340.13504247498283634No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT285300.122957867213261No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC261480.11269198429345773No Hit
TCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG246580.10627042025042376No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG241180.10394314200663964No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC234560.10109007127074134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC174700.039.76864645
GTTGATC330450.032.618836
GGTGTGC710300.032.5484477
GTGTGCT1025400.031.3579488
TGTGCTC981300.031.322799
CCTGGTT350200.030.9935252
TCGTATG225300.030.85713244
GTGCTCT990350.030.0756110
TTGATCC358150.030.0709047
AGCCATG358700.029.88738645
GGGTGTG381250.029.8245146
TAGCATA366150.029.49420520
CATATGC367550.029.35693623
CTGGTTG372750.029.2979133
GCAACAC105950.029.2587151
GTAGCAT369400.029.1935319
CGACCAT275100.029.17425517
TGATCCT369650.029.0988628
CCAGTAG371100.029.06660716
TTAAGCC369150.029.06499742
Produced by FastQC (version 0.11.2)