FastQCFastQC Report
Wed 17 Sep 2014
ANCMTT_4_01_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMTT_4_01_GCCAAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31351133
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA4857491.5493826012603755No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC1741930.5556194731463134No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG1523950.4860908854553997No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG1330640.4244312318792434No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG1276060.4070219726987219No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG959330.3059953208070662No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA945580.30160951439936795No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT939030.2995202757106099No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA715010.22806512287769634No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT609740.19448738902035853No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA595140.18983046003472986No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG583260.18604112329847855No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA549400.1752408756646849No Hit
TCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG510180.16273096095123582No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA499580.15934990292057388No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT498360.15896076227930903No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC472860.1508270849413959TruSeq Adapter, Index 6 (100% over 50bp)
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC468440.14941724753615762No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA466210.14870594947876367No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG461290.14713662820415452No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA423810.13518171735611598No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA421420.13441938446052332No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC398570.1271309716302757No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT370980.11833065171839244No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG334520.1067010879638704No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG321050.10240459252301981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC69850.033.65984745
GGTGTGC805700.033.287
TGTGCTC1033650.031.2647619
GTGTGCT1152850.030.8891898
GGGTGTG359800.029.4893976
GTGCTCT993050.029.35530510
GTTGATC276700.027.4665286
TAGGGTG66200.026.95064
CGGTGTG266100.026.5230146
GCTCTTC862050.025.95038212
TACGGTG39050.025.696094
TCGTATG92050.025.56651344
CCTGGTT306150.025.2446442
TGCTCTT867400.025.14945211
TTGATCC309550.024.6098757
GCAACAC263200.024.513431
GCGGTGT55350.024.1447145
TCTTCCG803450.024.12812814
TGATCCT324300.023.6362428
AGGTGTG169900.023.2665316
Produced by FastQC (version 0.11.2)