ANCMTT_24_02_CAGATC_L005_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ANCMTT_24_02_CAGATC_L005_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10532687
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC	192323	1.8259633083181908	No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA	93363	0.8864119858493849	No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC	74486	0.7071889632721451	No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA	42776	0.40612618603401013	No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG	42469	0.40321145022158167	No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA	36004	0.3418311015982911	No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	35685	0.33880243474433447	No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG	34746	0.3298873307447568	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	33840	0.3212855371093815	TruSeq Adapter, Index 7 (100% over 50bp)
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	29307	0.27824808617212304	No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG	28204	0.26777592460499394	No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG	25541	0.24249272763920546	No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT	24835	0.23578978469596598	No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG	20319	0.1929137360675391	No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC	20237	0.1921352072837634	No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA	18944	0.1798591375591053	No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG	17920	0.17013702201536987	No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC	15685	0.14891736553075205	No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACC	13498	0.1281534332122468	No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG	13076	0.12414685825184019	No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG	12941	0.12286513403464852	No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT	12408	0.11780469694010653	No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT	12212	0.11594382326181345	No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC	12030	0.11421586913196985	No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTC	11845	0.11245943224174419	No Hit
GAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTT	11477	0.1089655469682143	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATAAG	9730	37.668163	27
GTAACAT	9775	37.47405	33
AAGTAAC	9805	37.449047	31
CGTATGC	4400	37.430763	45
TATAAGT	9800	37.3991	28
TAAGTAA	9815	37.38779	30
ATAAGTA	9855	37.32736	29
TAGTATA	10200	35.975216	25
AGTAACA	10285	35.658737	32
TAACATG	10400	35.222668	34
AGTATAA	10465	35.108402	26
ATACCGT	27095	34.42171	2
TACCGTC	27245	34.344234	3
CGTCGTA	27610	34.085613	6
GTCGTAG	27795	33.95588	7
GTTAGTA	10770	33.9459	23
ACCGTCG	27815	33.72132	4
CGACCAT	28075	33.71451	17
TAGTTCC	28070	33.62337	11
GTAGTTC	28055	33.617134	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content