FastQCFastQC Report
Wed 17 Sep 2014
ANCMTT_24_01_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameANCMTT_24_01_GCCAAT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6706500
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGC3244764.838231566390815No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG757871.130052933721017No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGA499440.7447103556251399No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA473360.7058227093118616No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA431230.6430030567360024No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC430380.641735629613062No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAA288680.43044807276522773No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGG259200.38649071796018786No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA250540.3735778722135242No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA218610.3259673451129501No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGAT193640.2887348095131589No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA176160.26267054350257213No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGC169450.2526653246850071No Hit
AGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG169210.25230746290911804No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA161340.2405725788414225No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG155030.2311637963170059No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA148210.22099455751882505No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC145910.21756504883322147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA143340.2137329456497428No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG139150.2074852754790129No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACC138300.20621784835607246No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC132150.19704764034891523TruSeq Adapter, Index 6 (100% over 50bp)
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG129720.19342428986803847No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT122230.18225602027883397No Hit
GCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG118990.17742488630433162No Hit
CACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAA111490.1662417058077984No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG108790.16221576082904643No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA108490.16176843360918514No Hit
TCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC103730.1546708417207187No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT96720.1442182956832923No Hit
ACAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG96680.1441586520539775No Hit
GCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA94530.14095280697830462No Hit
GAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTT94410.1407738760903601No Hit
CCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG88490.1319466189517632No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGG87680.13073883545813764No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT87160.12996346827704466No Hit
CGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCG85840.1279952285096548No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC85720.1278162976217103No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG78290.11673749347647805No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT76860.11460523372847238No Hit
GACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAG75620.11275628121971222No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAG73790.11002758517855812No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT71280.10628494743905167No Hit
AGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTT70800.10556922388727354No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG70290.10480876761350928No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC69170.10313874599269365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT381600.041.77278517
TCCGACC381200.041.75759515
TTCCGAC382300.041.6315614
CCGACCA384050.041.51215716
GACCATA384600.041.43462818
CATAAAC385300.041.3117121
CCATAAA391250.040.7639620
ATAAACG391850.040.60393522
ACCATAA394450.040.43416619
ATACCGT403200.039.2964672
ACGATGC404600.039.27464726
TACCGTC404450.039.2555473
CGACCGG406000.039.22614333
AACGATG405200.039.21619825
CGGCGAT405800.039.19149837
GTAGTTC408000.039.1290410
TAGTTCC408150.039.12626611
GTCGTAG408850.039.1192257
CGTCGTA408350.039.101016
TAAACGA409400.038.8688423
Produced by FastQC (version 0.11.2)