FastQCFastQC Report
Wed 17 Sep 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867264
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36100.041.5942151
ATCGGAA35950.041.3724632
ACACGTC31150.039.94938713
TCGGAAG37250.039.5068473
CGGAAGA37250.039.26434
CGTCTGA31500.038.8744716
ACGTCTG31650.038.76109315
CACGTCT32550.038.10276814
CACACGT33500.037.7454712
GCACACG34700.036.8001911
AATTCGG3950.032.48112523
AGAGCAC45400.030.0450138
GAACTCC39150.029.8306921
TTCGGTA4550.029.16832725
AGCACAC45100.029.10889810
CTGAACT42950.028.20519819
GAATTCG4600.027.89139722
TGAACTC43800.027.2729220
TATTCGT3950.027.233881
GAGCACA49800.027.0756029
Produced by FastQC (version 0.11.2)