FastQCFastQC Report
Wed 17 Sep 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832196
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36750.041.8293881
ATCGGAA37050.041.3993382
ACACGTC32950.041.35350813
CGTCTGA33600.039.9569416
ACGTCTG33950.039.80908615
TCGGAAG38850.039.193043
CGGAAGA38800.038.9545864
CACGTCT34850.038.90964514
CACACGT35850.038.56095512
GCACACG37050.037.54963311
AGCACAC46450.030.86363810
CTGAACT43050.030.67893619
GAACTCC41250.030.59704421
AGAGCAC48050.030.4303078
CGCAATA2900.030.38553633
AATTCGG3300.027.95562423
GAGCACA51550.027.842999
TTCGGTA3300.027.28399325
TGAACTC47500.027.26343720
GAAGAGC55700.026.8941296
Produced by FastQC (version 0.11.2)