FastQCFastQC Report
Wed 17 Sep 2014
SV49_GCCAAT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV49_GCCAAT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32468373
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3027080.9323165038174226TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA353350.042.909181
ATCGGAA355350.042.7005422
CGCCAAT344450.042.20602433
TCTCGTA344900.042.09775541
CTCGTAT345300.042.0685442
TATCTCG347200.042.05221639
TCGTATG347850.041.87085343
CGTCTGA355100.041.862316
GTCACGC352100.041.65485829
CGTATGC348700.041.63039844
ACACGTC358500.041.57184613
TCGGAAG366250.041.404453
ACGCCAA351300.041.38945432
ACGTCTG360600.041.29867615
ATCTCGT356450.040.9293840
CGGAAGA371300.040.7790874
TCACGCC361300.040.5758130
AGTCACG361350.040.56365628
CACGTCT372000.040.00887714
CACGCCA365200.039.95293431
Produced by FastQC (version 0.11.2)