FastQCFastQC Report
Wed 17 Sep 2014
SV48_ACAGTG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV48_ACAGTG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30544144
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC339520.111157150123441TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT49400.034.1655442
TCTCGTA50500.033.37677841
TCGTATG52750.032.11924743
CGTATGC54100.031.3990344
ATCTCGT57150.029.57179540
GATCGGA60900.029.0555111
ACACGTC60300.028.99132713
GATCTCG58650.028.77711339
ATCGGAA62950.028.271492
CGTCTGA62550.028.09267416
ACGTCTG66450.026.37617715
CACGTCT74900.023.55068814
TCGGAAG76900.023.4937043
CGGAAGA82300.022.0608254
GTATGCC79150.021.80230745
CACACGT90550.019.80243512
GCACACG93350.019.11203411
AGTGATC98800.017.60672836
GTGATCT107350.016.01575537
GAACTCC127550.014.11103921
Produced by FastQC (version 0.11.2)