FastQCFastQC Report
Wed 17 Sep 2014
SV46_CGATGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV46_CGATGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30185909
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA27400.026.52455534
CTCGTAT27200.026.47514542
TCTCGTA27250.026.34398341
TATCTCG27900.026.0528739
CACCGAT29000.025.29230131
TCGTATG28500.025.18508143
ACCGATG29050.025.17290532
CCGATGT32000.022.64133333
CGTATGC32050.022.39398844
GATCGGA38350.020.3713261
CGTCTGA38600.020.34255216
ACACGTC39100.020.13979313
ATCTCGT35900.020.12184740
ATCGGAA39650.019.9176332
GTCACCG38600.019.00123429
TCACCGA39300.018.31945430
ACGTCTG43150.018.19750815
CACGTCT48600.016.29572714
TCGGAAG51750.015.6949483
CGGAAGA54100.015.1374014
Produced by FastQC (version 0.11.2)