FastQCFastQC Report
Wed 17 Sep 2014
SV45_CAGATC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV45_CAGATC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34045809
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3167350.9303200872683038TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA369400.042.721411
ATCGGAA373050.042.332972
CTCGTAT358950.042.25562342
TCTCGTA359450.042.19684641
CGTCTGA368700.041.91096516
CGTATGC362400.041.81948544
TCGTATG363250.041.81165743
ACACGTC370850.041.7890813
ACGTCTG372200.041.56521215
TCGGAAG382100.041.22363
CGGAAGA383750.040.9862144
ATCTCGT374150.040.77955640
CATCTCG373200.040.73864439
CACGTCT381850.040.56192814
GCACACG389800.039.9057711
CACACGT393600.039.4463112
GTATGCC386500.039.29847745
GATCATC408550.037.35681536
GAACTCC437600.035.31017721
AGTCACC435050.035.238328
Produced by FastQC (version 0.11.2)