FastQCFastQC Report
Wed 17 Sep 2014
SV30_CAGATC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV30_CAGATC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19463103
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2999141.5409362011802537TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT331800.042.8226442
TCTCGTA331800.042.80907441
GATCGGA343600.042.7736661
TCGTATG334300.042.56976743
ATCGGAA346100.042.5284352
CGTATGC334750.042.48478744
ACACGTC342150.042.4595913
CGTCTGA341900.042.2866416
ACGTCTG344450.042.04543715
TCGGAAG351150.041.7683833
CATCTCG341150.041.6820739
CGGAAGA351750.041.651724
ATCTCGT342950.041.61411740
CACGTCT351350.041.33498814
GCACACG353250.041.16298311
GTATGCC347850.040.87845245
CACACGT356150.040.8409712
GATCATC359300.039.5577236
GAACTCC376950.038.2584121
CCAGATC376050.037.94144433
Produced by FastQC (version 0.11.2)