FastQCFastQC Report
Wed 17 Sep 2014
SV29_GCCAAT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV29_GCCAAT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27909651
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC6853302.455530525981855TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA766250.044.1155781
ATCGGAA771200.043.946072
ACACGTC767750.043.5324313
TCGGAAG778300.043.4772073
CGTCTGA767400.043.47019216
CGCCAAT753450.043.3590433
ACGTCTG771950.043.27517315
TCTCGTA758300.043.24590741
CGGAAGA783050.043.1727074
CTCGTAT759900.043.1726242
GTCACGC762850.043.14581329
TATCTCG761800.043.12432539
ACGCCAA758000.043.09869832
TCGTATG763450.043.04643643
CGTATGC763550.043.00451744
ATCTCGT767000.042.79644440
CACGTCT783150.042.7137514
AGTCACG770400.042.70799328
TCACGCC768500.042.6880830
CACGCCA769500.042.5416331