FastQCFastQC Report
Wed 17 Sep 2014
SV28_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV28_ACAGTG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21604389
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC481460.22285286568391266TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT62050.036.88187842
TCTCGTA63100.036.33947441
TCGTATG63800.035.90051343
CGTATGC66300.034.61442644
ATCTCGT66900.034.47716540
GATCTCG67700.034.1363139
CGTCTGA71900.032.76259216
ACACGTC73200.032.30368413
GATCGGA75950.031.776271
ATCGGAA76600.031.6349012
ACGTCTG76350.030.94145215
CACGTCT82700.028.70168514
TCGGAAG85800.028.4255893
CGGAAGA89000.027.5293084
GTATGCC85750.027.15668145
CACACGT91100.026.37626612
GCACACG95450.025.00914811
AGTGATC98550.023.51881436
GTGATCT102200.023.07518637
TGATCTC118000.020.08080938
Produced by FastQC (version 0.11.2)