FastQCFastQC Report
Wed 17 Sep 2014
SV27_TGACCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV27_TGACCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38694961
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5624441.4535329290033399TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT634750.042.29122542
TCGTATG634350.042.28285243
CGTATGC640200.041.82510444
TCTCGTA642450.041.80186541
ATCGGAA676900.041.5855942
ATCTCGT648900.041.45918340
GATCGGA677050.041.4577031
ACACGTC665100.041.40513613
AATCTCG652350.041.3158239
CGTCTGA668650.041.07095316
GTATGCC652150.041.0656645
ACGTCTG674250.040.80658715
CACGTCT685400.040.2051214
CACACGT688750.040.0715312
GACCAAT673300.039.8385135
ACCAATC679750.039.4617236
TCGGAAG713300.039.440013
CGGAAGA724900.038.8113824
CCAATCT695450.038.63557437
CAATCTC700200.038.3927638
Produced by FastQC (version 0.11.2)