FastQCFastQC Report
Wed 17 Sep 2014
SV26_CGATGT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV26_CGATGT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43829123
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA56450.031.4894534
CTCGTAT58100.030.52092242
TATCTCG58500.030.42766639
CACCGAT59350.030.13990631
TCGTATG59150.030.01268643
TCTCGTA59350.029.9160241
ACCGATG62150.028.78229932
CGTATGC63150.028.28905144
CCGATGT64300.027.61006533
ATCTCGT68250.026.21271540
GATCGGA75150.025.7631171
CGTCTGA73600.025.52510316
GTCACCG71200.025.34263229
TCACCGA72000.025.1860430
ATCGGAA78400.025.0823422
ACACGTC76250.024.84454513
ACGTCTG82300.023.10019915
CACGTCT91100.021.01696614
TCGGAAG95750.020.8658223
CGGAAGA102550.019.306364
Produced by FastQC (version 0.11.2)