FastQCFastQC Report
Wed 17 Sep 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4322247
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT32400.010.5816622
CTTTGTG32250.010.5642081
GTGTTTG37300.010.0916625
GACGGGC4458.731149E-1110.0700649
TTGTGTT37200.09.8212721
ACGGGCG3901.0606527E-89.76678410
TGTGTTT40300.09.396024
TGTTTGA44500.08.4588556
CGGTGTG6205.482434E-97.59093715
TTTGAGG11900.07.34310778
TGTGTAC7751.1295924E-96.940525518
GGGACGT7503.588866E-96.922148733
CAGGGAC7201.15942385E-86.896587431
GGGCAGG7351.7789716E-86.750019628
GCGGTGT6651.580247E-76.740095614
CGTAATC8801.4737452E-86.15864537
CAAAGGG8151.3854151E-76.083120324
GTGTACA8505.360198E-86.07119919
GGCAGGG8606.062146E-86.034648429
GCAGGGA8452.7200804E-75.876244530
Produced by FastQC (version 0.11.2)