FastQCFastQC Report
Wed 17 Sep 2014
handrnoggin15hpf2_AAGACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrnoggin15hpf2_AAGACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30117555
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT195700.012.3137622
TTGTGTT288000.011.8485951
GTGTTTG294450.011.6522943
TGTGTTT311250.011.03156952
TGTTTGA374550.09.1543364
GACGGGC39850.08.5251179
CTTTGTG290650.08.5111351
TGACGGG53100.08.38923558
CTACCCG22100.08.34810216
GAGGCTA21500.07.95302912
ACGGGCG40550.07.656746410
AGGCTAC23650.07.515572513
TTTGAGG102100.07.00736143
TTGACGG69300.06.98002437
CGGTGTG55300.06.713127615
GCTACCC26550.06.69466315
GCGGTGT55400.06.619786314
TGTGTAC73800.06.554543518
TCCCCCT33900.06.37173743
TAACTTT184200.06.342171
Produced by FastQC (version 0.11.2)