FastQCFastQC Report
Wed 17 Sep 2014
handrlaz15hpf2_TTCAGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrlaz15hpf2_TTCAGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24197825
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT179550.014.1859512
TTGTGTT271000.012.0855191
GTGTTTG278550.011.8812253
TGTGTTT292750.011.2599952
CTTTGTG254900.010.2172761
TGTTTGA363750.09.4866041
TGACGGG48550.08.8510348
GACGGGC36550.08.3099099
ACGGGCG35600.07.96310
CTACCCG22900.07.95827913
TTGACGG58600.07.9089627
AACTTTG144700.07.3862252
TTTGAGG94600.07.3726273
TAACTTT153200.07.27307461
GAGGCTA21550.07.203828312
GCGGTGT49800.07.093162514
GCTACCC25900.07.036339812
TGTGTAC67150.07.0026718
GTGTACA69450.06.86786219
CGGTGTG51900.06.762805515
Produced by FastQC (version 0.11.2)