FastQCFastQC Report
Wed 17 Sep 2014
handrlaz15hpf1_AAGGGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrlaz15hpf1_AAGGGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22653397
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT204300.013.6568892
CTTTGTG252350.011.2305271
TTGTGTT298500.010.9270221
GTGTTTG303800.010.89373
TGTGTTT322000.010.3000672
TGTTTGA405150.010.2730361
TAACTTT143550.09.3929371
TTTGAGG105850.08.3319323
AACTTTG160000.08.1706122
GTTTGAG279650.07.587442
TTTGAGT68600.07.4775833
TGACGGG46900.07.4354868
GACGGGC37700.07.3403079
GAGGCTA28500.07.183839312
GAGGGGC23950.07.13934859
CTACCCG29700.07.04519916
TTTGAGC61950.06.7912673
ACGGGCG36500.06.780411710
GCTACCC31800.06.721454615
TTGACGG58250.06.7205357
Produced by FastQC (version 0.11.2)