FastQCFastQC Report
Wed 17 Sep 2014
handrdnfgfr15hpf2_CCTCGG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrdnfgfr15hpf2_CCTCGG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29995282
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT288950.011.29110052
GTGTTTG403300.09.9745013
TTGTGTT413700.09.8102331
CTTTGTG339500.09.5674391
TGTGTTT443700.09.1837482
TGTTTGA511550.08.3297431
GTTTGAG285300.07.9812282
TAACTTT209500.07.9616541
AACTTTG218850.07.54641152
GACGGGC45250.07.25915589
TGACGGG68700.06.975498
ACGGGCG43850.06.823976510
GAGGCTA29250.06.61499512
CTACCCG31250.06.55171316
TTGACGG82000.06.310537
TTTGAGG143850.06.27171563
TTTGAGT111350.06.04133373
TTTGACG142800.05.98697236
AGGCTAC35400.05.783625613
GTCCCCC29900.05.6449242
Produced by FastQC (version 0.11.2)