FastQCFastQC Report
Wed 17 Sep 2014
handrdnfgfr15hpf1_GGACCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrdnfgfr15hpf1_GGACCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26693275
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT196100.013.98742
TTGTGTT299350.012.3697781
GTGTTTG308000.012.0308183
TGTGTTT319950.011.5971012
CTTTGTG285850.09.95369151
TGTTTGA399950.09.2592634
TGACGGG54200.07.6378458
TAACTTT173150.07.30615851
AACTTTG168350.07.2844082
TTTGAGG112950.07.27041056
TTGACGG67000.06.95100937
TTTGAGT67650.06.85096453
GAGGCTA25800.06.71472512
GACGGGC41000.06.5849089
ACGGGCG38500.06.545023410
GTTTGAG301900.06.31293342
CTACCCG28300.06.280693516
GAGGGGC27250.06.19222979
TGAGGGG42700.06.16467678
TGTGTAC67100.06.035528718
Produced by FastQC (version 0.11.2)