FastQCFastQC Report
Fri 31 Oct 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4621995
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC282900.6120733579331004No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC69890.15121176028965846No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA49340.10675044001562095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG47800.041.6756911
GTCTCCC42050.041.2575442
CAGGCGG41200.041.25107636
CACACGT49000.040.63300312
ACACGTC49150.040.55380613
GGTCTCC42500.040.48497841
TCGGGGA41200.040.29480417
CCAGGCG41550.040.16063735
CGTCTGA49500.040.08865416
CGGTCTC43000.040.0309940
CTCGGGG32100.039.93188516
CCCAGGC42150.039.76207434
CACGTCT50150.039.74515514
GATCGGA52100.039.704481
CCTCGGG32400.039.6305515
ACGTCTG50500.039.51330615
AGCACAC50800.039.34111410
GAACTCC50300.038.89853321
AGAGCAC52300.038.5377858
GGCGGTC44550.038.4504538
Produced by FastQC (version 0.11.2)