FastQCFastQC Report
Fri 31 Oct 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5383669
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG119770.22246910053348376TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG118460.22003581572344064TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCCATCTCGTATGCCG117310.2178997260047005TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC91620.17018133915736647TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG301700.043.1897911
ACACGTC301150.043.0239113
CACACGT302450.042.99503312
ACGTCTG299650.042.96575515
CACGTCT300700.042.94059414
AGCACAC305700.042.8242310
CGTCTGA299200.042.77937716
AGAGCAC308750.042.7517978
GATCGGA311450.042.7287521
GAACTCC292950.042.7103521
CTCCAGT267650.042.3658324
TCGGAAG316750.042.337533
CGGAAGA316400.042.3145564
CCAGTCA243050.042.2943926
CAGTCAC223250.042.1129327
CTGAACT302900.042.0734719
AACTCCA291400.041.9488122
GTCACGG52650.041.908729
ATCGGAA319650.041.8639952
TGAACTC304050.041.7342820
Produced by FastQC (version 0.11.2)