FastQCFastQC Report
Fri 31 Oct 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086471
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT186600.043.45553212
CACGTCT190350.042.93882814
GCACACG189650.042.92111611
ACACGTC189100.042.9163713
ACGTCTG191400.042.8080215
CGTCTGA189000.042.69086516
AGAGCAC193450.042.1266178
GATCGGA202800.042.009321
GTCTGAA188900.041.9854817
GAACTCC176150.041.8556421
AGCACAC192400.041.8451810
GAGCACA194100.041.837099
CCAGTCA144500.041.832526
CTGAACT187400.041.63553219
CGGAAGA200400.041.5989464
ATCGGAA202900.041.5842632
TCCAGTC153450.041.57760225
CAGTCAC134850.041.54483427
ACTCCAG167250.041.40890523
AACTCCA170150.041.32436422
Produced by FastQC (version 0.11.2)