FastQCFastQC Report
Fri 31 Oct 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919602
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC46900.16063833358108398TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC39780.13625144797133307TruSeq Adapter, Index 13 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG184600.043.15770711
CACACGT184600.043.08569712
ACGTCTG188400.042.70696615
ACACGTC186800.042.58812713
CACGTCT188600.042.5559914
CGTCTGA185750.042.38624616
CTGAACT181400.042.32856419
GTCTGAA184100.042.2375517
GATCGGA198650.042.1256371
TCTGAAC182800.042.03410718
AGAGCAC190800.041.967098
CGGAAGA196450.041.6025164
GAACTCC176100.041.57825521
GAGCACA193300.041.3763289
AACTCCA169900.041.27342222
ATCGGAA200550.041.2620162
CAGTCAC141500.041.15617827
AGCACAC192050.041.08020410
AGTCACC42600.040.86633328
TCCAGTC162350.040.7654625
Produced by FastQC (version 0.11.2)