FastQCFastQC Report
Fri 31 Oct 2014
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6147570
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG270820.44053178735663034TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG265740.43226835969334226TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC126090.2051054319023614TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGGATCTCGTATGCCG124780.20297450862698596TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC99650.16209656823753124TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT357550.043.23995212
GCACACG361300.042.92605211
ACGTCTG359650.042.77805315
CACGTCT360800.042.6846814
CGTCTGA359650.042.57785416
ACACGTC365000.042.30883413
CAGTCAC295350.042.17534627
GTCTGAA363900.042.10072317
CTGAACT362300.042.08571619
GATCGGA368550.042.004821
CGGAAGA374900.041.6503754
ATCGGAA373300.041.410922
TCTGAAC369050.041.40440818
CCAGTCA319150.041.24881726
AGAGCAC380900.041.1854868
AACTCCA361450.041.16814422
GAGCACA381650.041.060919
GAACTCC368300.041.05328821
TCCAGTC330900.040.88202725
ACTCCAG349900.040.62000323
Produced by FastQC (version 0.11.2)