FastQCFastQC Report
Fri 31 Oct 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496555
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC154790.34424131362787735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41800.041.58296211
ACACGTC42300.040.8815213
CACACGT42550.040.6413212
GATCGGA44700.040.6071931
ACGTCTG43000.040.05043815
CACGTCT42950.039.99905814
AGCACAC44600.039.47010410
GTCTCCC23000.039.26937542
CCAGGCG22850.039.20369335
CGTCTGA43900.039.17880616
TCGGGGA22600.038.9152317
AGAGCAC45700.038.811518
CAGGCGG23050.038.7857536
CCTCGGG19200.038.606415
CCCAGGC23050.038.55522534
CGGTCTC23400.038.4823940
CTCGGGG19300.038.4063616
TCGGAAG47350.038.338323
GAACTCC44150.037.6633621
ATCGGAA48750.037.2638742
Produced by FastQC (version 0.11.2)