FastQCFastQC Report
Fri 31 Oct 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511675
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC132670.5282132441498204No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC31940.12716613415350314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC30390.12099495356684285TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCC25400.041.9088142
CTCGGGG21200.041.7700816
CCAGGCG24900.041.69435
GCACACG35600.041.55809411
TCGGGGA26250.041.55715617
CGGTCTC25650.041.5403240
CAGGCGG25600.041.52544436
CCTCGGG21400.041.27651615
GGTCTCC26000.041.1572241
CACACGT35850.041.0974212
ACACGTC36050.041.0524213
CGTCTGA36850.040.98976516
ACGTCTG37750.040.94848615
TCGGAAG38400.040.6522373
CCCAGGC25150.040.63336634
GATCGGA38900.040.535661
GCGGTCT26800.040.33711239
CACGTCT37650.040.24652514
TCCTCGG22400.039.83846314
GGCGGTC27150.039.544738
Produced by FastQC (version 0.11.2)