FastQCFastQC Report
Fri 31 Oct 2014
MK203_GTGGCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK203_GTGGCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820721
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC181070.6419280744178527TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC32650.040.58670433
GCACACG40850.038.82833511
ACGTCTG41450.038.53904315
CACACGT41000.038.52302612
CACGTCT41400.038.31385814
GTATGCC33800.038.22157745
CGTCTGA41900.038.01774616
TCACGTG37500.037.61733230
CTGAACT42250.036.74295419
GTGGCCA34550.036.66165534
AGCACAC42800.036.37592710
ACACGTC43700.036.14288313
GTCACGT39300.035.894429
CGTATGC37100.035.85312344
TCTGAAC43100.035.81079518
GGCCATC35500.035.30031636
GCCATCT35750.035.2478837
CTCGTAT37850.034.96430242
AGTCACG40550.034.89949828
CAGTCAC41700.034.4226327
Produced by FastQC (version 0.11.2)