FastQCFastQC Report
Fri 31 Oct 2014
MK202_AGTTCC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK202_AGTTCC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5874597
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG166070.28269173187539504TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT30500.033.78277612
TATCTCG24900.033.7167941
GCACACG31650.032.6263611
ACGTCTG32050.032.1492515
CGTCTGA32250.032.01963416
GTCACAG27550.031.11501729
TCACAGT28100.030.66561730
CACGTCT33900.030.26206214
CGTATCT28200.029.68507439
CTGAACT35050.029.39828119
CACAGTT30400.028.27079231
AGTCACA30950.027.91521328
TCTGAAC36800.027.8779918
GTATCTC30650.027.75461440
AGCACAC37800.027.7347210
GTCTGAA38250.026.99694617
CTCGTAT32550.026.1405444
CAGTCAC37400.025.8682627
CCGTATC32700.025.66791338
GCCCTAT450.001225955625.0041961
Produced by FastQC (version 0.11.2)