FastQCFastQC Report
Fri 31 Oct 2014
MK201_CAGATC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK201_CAGATC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072783
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT3100.023.2260191
CACGTCT5750.021.12909914
CGTCTGA5600.020.89153516
CTATACG2200.020.4532515
ACGTCTG6050.020.08137515
CACACGT6000.019.87374112
GCACACG6000.019.49876611
CCGGGTT2100.019.285891
CGCCCGT1051.4465877E-619.285891
CGCGGCT2500.018.9001711
ACCTATA2400.018.74881613
CACCTAT2550.018.52823812
ATACGCC2550.018.52823817
CGGGGCT2800.018.4823111
AGTCACC4150.018.4325728
TATACGC2550.017.6459416
CCGGCCC1153.4534569E-617.60758218
AACTTAC3000.017.2489076
ACTTACA2900.017.0678847
CTGAACT7650.017.05774519
Produced by FastQC (version 0.11.2)