FastQCFastQC Report
Fri 31 Oct 2014
MK200_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK200_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928355
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC279950.404064168189996TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC39650.037.1685828
GTCACCT42050.034.9931729
GTATGCC43450.034.45167545
CACACGT46750.033.54146212
ACGTCTG47400.032.74926815
TGTAATC44350.032.36554736
CGTCTGA48050.032.30648416
GCACACG48550.032.29790511
CGTATGC46650.032.23319244
GTAATCT44900.031.82129137
CTCGTAT47650.031.41501442
CACGTCT49500.031.26900514
CTGAACT50250.030.84796519
GTCTGAA51350.030.4055617
TCTGAAC52300.030.19717218
TCGTATG50350.029.95393243
TCACCTT51450.028.81726830
CTTGTAA50600.028.810834
AGCACAC54900.028.72567610
TTGTAAT52300.028.1338335
Produced by FastQC (version 0.11.2)